Engineering Salidroside Biosynthetic Pathway in Hairy Root Cultures of Rhodiola crenulata Based on Metabolic Characterization of Tyrosine Decarboxylase
نویسندگان
چکیده
منابع مشابه
Engineering Salidroside Biosynthetic Pathway in Hairy Root Cultures of Rhodiola crenulata Based on Metabolic Characterization of Tyrosine Decarboxylase
Tyrosine decarboxylase initializes salidroside biosynthesis. Metabolic characterization of tyrosine decarboxylase gene from Rhodiola crenulata (RcTYDC) revealed that it played an important role in salidroside biosynthesis. Recombinant 53 kDa RcTYDC converted tyrosine into tyramine. RcTYDC gene expression was induced coordinately with the expression of RcUDPGT (the last gene involved in salidros...
متن کاملThe c4h, tat, hppr and hppd Genes Prompted Engineering of Rosmarinic Acid Biosynthetic Pathway in Salvia miltiorrhiza Hairy Root Cultures
Rational engineering to produce biologically active plant compounds has been greatly impeded by our poor understanding of the regulatory and metabolic pathways underlying the biosynthesis of these compounds. Here we capitalized on our previously described gene-to-metabolite network in order to engineer rosmarinic acid (RA) biosynthesis pathway for the production of beneficial RA and lithospermi...
متن کاملHairy Root Cultures
Hairy root culture plays an important role in investigation of alkaloid production in culture in vitro. The complexity of scientific work and the production capacity limitations associated with whole plant systems and unorganized cells can be overcome using culture of hairy roots. This paper presents an example of a strategy to produce secondary metabolites from plants, in which we investigated...
متن کاملEffect of Salicylic Acid on Antioxidant Activity in Milk Thistle Hairy Root Cultures
Background: Elicitors can trigger defense responses and activate specific genes involved in secondary metabolite biosynthesis. Objective: For understanding SA-treatment signaling pathway we determined defense-related compounds and enzymes in S. marianum hairy root cultures. Methods: 28 day old hairy roots were treated by salicylic acid (6 mg/50 ml culture) for different times (24, 48, 72, 96...
متن کاملProtective Effects of a Rhodiola Crenulata Extract and Salidroside on Hippocampal Neurogenesis against Streptozotocin-Induced Neural Injury in the Rat
Previously we have demonstrated that a Rhodiola crenulata extract (RCE), containing a potent antioxidant salidroside, promotes neurogenesis in the hippocampus of depressive rats. The current study was designed to further investigate the protective effect of the RCE on neurogenesis in a rat model of Alzheimer's disease (AD) induced by an intracerebroventricular injection of streptozotocin (STZ),...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0075459